Abstract: Inflammatory bowel diseases(IBD) mainly include ulcerative colitis(UC) and Crohn’s disease(CD), affecting about six to eight million people around the world. Features of IBD include chronicity, progressiveness and recurrence, obviously affecting patients’ quality of life and increasing medical expense. IBD is usually considered to be derived from abnormal immune response of genetic suscept to microorganism. …
Abstract: Natural killer cells(NK cells) are important effector cells in innate immune system, and their roles in anti-virus and anti-tumor immunity are also significant. High purity NK cells are usually separated from peripheral blood mononuclear cell via density gradient centrifugation and magnetic-activated cell sorting(MACS). Cell amplification in vitro via IL-2 or IL-15 maintains activity and …
Abstract: Atherosclerosis is the chronic vascular disease induced by deposited substances(e.g. fat and cholesterol in artery wall). Formed plaque stiffens and narrows artery to block blood flow. Causes of atherosclerosis development are complex. The inflammation indicates important roles of inflammatory cells and factors. Keywords: Atherosclerosis, Chronic Vascular Disease, Inflammation, Plaque 1. Risk Factors in Atherosclerosis …
Abstract: Fluorescent proteins(FPs) are derived from marine organisms(e.g. jellyfish, coral etc). They can realize autofluorescence without exogenous substrates. Their roles in modern life science research are very important. Wide applications of fluorescent proteins in flow cytometry include labeling, gene expression tracking, protein localization and cellular dynamics. This article presents fluorescent proteins from basic features, type …
Abstract: HER2 is the transmembrane glycoprotein of epidermal growth factor receptor family, involved in cell proliferation and differentiation. Abnormal expression or mutation is closely related to tumor invasiveness and poor prognosis. This article summarizes HER2 targeted therapy, biological characteristics, signaling pathway and regulatory mechanism. Keywords: HER2 Targeted Therapy, HER2 Protein Function, HER2 Signaling Pathway 1. …
Abstract: During flow cytometry analysis of immunology, CD45 marker is nearly used in each antibody combination. As the common antigen of leukocytes, CD45 is widely expressed on the surface of hematopoietic derived leukocytes. Roles in differentiating immune cells and non-immune cells are very important. CD45 is the gating marker required for immunophenotype, tumor microenvironment analysis …
Abstract: Fatty liver refers to excessive fat accumulation in the liver, accounting for more than 5% of liver weight. Changes in diet, less exercising and metabolic diseases(e.g. obesity, hypertension, diabetes etc) increase. The incidence of fatty liver disease(FLD) increases annually. FLD has become one of common hepatopathies worldwide. Early symptoms of most patients are not …
Abstract: After data collection, scientific gating strategy for flow cytometry analysis is required to recognize abnormal cell population in the sample. Data analysis include single and multi-parameter analysis. Important roles of gating in selecting target cell population can remove interference. Accurate identification of cellular phenotype and function provides reliable evidence for disease diagnosis and evaluation. …
Abstract: Pulmonary hypertension(PAH) is the pulmonary arteriole disease featured with persistent vasoconstriction, thickening and remodeling. The increased pulmonary vascular resistance causes right heart failure and even death. Normal pulmonary artery pressure is lower than systemic circulation pressure. If mPAP in resting conditions is above 25 mmHg, pulmonary hypertension is definitely diagnosed. Keywords: Pulmonary Hypertension, Increased …
Abstract: Flow cytometry detects cell surface markers(e.g. CD molecules) via fluorescence-labeled antibodies, and is widely applied in typing and functional analysis of immune cells. Besides surface flow cytometry staining, quantitative analysis can be performed for intracellular or nucleus cytokines, functional proteins and DNA content using fixation and permeabilization technology. Membrane permeability of different dyes is …
