Lysosome
Lysosomes are shaped variously and vesicular structures (0.025 to 0.8 micrometers in size) containing numerous hydrolytic enzymes.
Their intracellular function is to break down external substances that have entered the cell, as well as to digest portions of the cell's own cytoplasm or organelles. LAMP1 and LAMP2 are two transmembrane proteins located on the lysosomal membrane and can protect the protein from being hydrolyzed via their amino termini highly glycosylated on the lumenal surface of the lysosome. They are commonly used as lysosomal marker proteins.
Three main functions of lysosomes:
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Endocytosis Fuse with food vacuoles to absorb nutrition |
Phagocytosis Phagocytose harmful substances or pathogens from the extracellular environment |
Autophagy Phagocytoseaged or damaged organelles and other biologically unnecessary intracellularmacromolecules |
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Catalog No. |
Product Name |
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anti- LAMP1 antibody |
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anti- LAMP1 antibody |
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anti- LAMP1 antibody |
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anti- LAMP2 antibody |
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anti- LAMP2 antibody |
Endosome
Endosomes are acidic vesicular structures formed by membrane wrapping during endocytosis and don't contain lysosomal enzymes.
There are early endosomes, late endosomes and recycling endosomes based on different stages. While the endocytosis happens, the vesicle will be released. Then vesicle will fuse with early endosomes (markers such as RAB5, RAB4, transferrin and its receptor, and EEA1), and then mature into late endosomes (markers such as RAB7, RAB9, and mannose 6-phosphate receptor), and fuse with lysosomes for degradation or recycling (marker RAB11) back to the cell membrane.
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Catalog No. |
Product Name |
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anti- RAB4A antibody |
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anti- RAB5A antibody |
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anti- RAB5A-Specific antibody |
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anti- RAB7A antibody |
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anti- RAB9A antibody |
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anti- RAB9A-Specific antibody |
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anti- RAB11 antibody |
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anti- RAB11-Specific antibody |
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anti- CD63 antibody |
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anti- EEA1 antibody |
Autophgosome
Autophagosome refers to vesicles formed by the membrane (derived from the ER or Golgi complex membrane) wrapping of intracellular components including damaged organelles or excessively stored substances such as glycogen due to physiological or pathological reasons.
Two common markers for autophagosome formation include ATG12 complex which binds to the autophagic vesicles and LC3 protein which associates with the autophagic vesicles to form autophagosomes.
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Catalog No. |
Product Name |
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anti- ATG12 antibody |
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anti- ATG12 antibody |
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anti- ATG12 antibody |
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anti- P62/SQSTM1 antibody |
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anti- P62/SQSTM1 antibody |
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anti- P62/SQSTM1 antibody |
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anti- Cleaved LC3 antibody |
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anti- LC3 antibody |
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anti- LC3A-Specific antibody |
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anti- LC3B-Specific antibody |
