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CASP8 antibody

Category:

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Metabolism

Synonyms：	Caspase-8 (CASP-8)\|Apoptotic cysteine protease\|Apoptotic protease Mch-5\|CAP4\|FADD-homologous ICE/ced-3-like protease\|FADD-like ICE (FLICE)\|ICE-like apoptotic protease 5\|MORT1-associated ced-3 homolog (MACH)\|Caspase-8 subunit p18\|Caspase-8 subunit p10\|CASP8\|MCH5 antibody
Catalogue No.：	FNab01294	Reactivity：	Human
Host：	Mouse	Tested Application：	ELISA, IHC, WB,IF,IP
Clonality：	monoclonal	Isotype：	IgG1

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: CASP8 antibody
Catalogue No.: FNab01294
Size: 100μg
Form: liquid
Purification: Protein A+G purification
Purity: ≥95% as determined by SDS-PAGE
Clonality: monoclonal
Isotype: IgG1
Clone ID: 2C2
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: caspase 8, apoptosis-related cysteine peptidase
Alternative Names: Caspase-8 (CASP-8)|Apoptotic cysteine protease|Apoptotic protease Mch-5|CAP4|FADD-homologous ICE/ced-3-like protease|FADD-like ICE (FLICE)|ICE-like apoptotic protease 5|MORT1-associated ced-3 homolog (MACH)|Caspase-8 subunit p18|Caspase-8 subunit p10|CASP8|MCH5 antibody
UniProt ID: Q14790
Observed MW: 50-55 kDa, 25-32 kDa

Application

Tested Applications: ELISA, IHC, WB,IF,IP
Recommended dilution: WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:100-1:400; IF: 1:50-1:500

Validated Images

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab01294(CASP8 antibody) at dilution of 1:1000

IP Result of anti-Caspase 8 (IP:FNab01294, 5ug; Detection:FNab01294 1:500) with HepG2 cells lysate 2400ug.

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab01294(Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab01294 ( Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Background: Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro-and cleaved-caspase 8.

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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