MCF7 cells were subjected to SDS PAGE followed by western blot with FNab10534(NES antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain using FNab10534(NES antibody) at dilution of 1:50
Immunofluorescent analysis of SH-SY5Y cells using FNab10534(NES antibody) at dilution of 1:50.
Background
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.