Products
γ-H2AX antibody
- Product Name
- γ-H2AX antibody
- Catalogue No.
- FNab10441
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- A synthetic phosphorylated peptide around S139 of human H2AX
- Alternative Names
- gamma H2A.X antibody, γH2AX antibody, Phospho-H2AX-S139 antibody
- UniProt ID
- P16104
- Observed MW
- 17 kDa
Application
- Tested Applications
- WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab10441(γ-H2AX Antibody) at dilution of 1:1000. NIH/3T3 cells were treated by UV for 15-30 minutes.
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using FNab10441(γ-H2AX Antibody) at dilution of 1:100
Immunofluorescent analysis of HeLa cells using FNab10441 (γ-H2AX antibody) at dilution of 1:100. HeLa cells were treated by UV for 15-30 minutes at RT.
- Background
- H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
