Products

HIF1a antibody

Synonyms:BHLHE78 antibody, MOP1 antibody, PASD8 antibody
Catalogue No.:FNab10340Reativity:Human, Mouse
Host:RabbitTested Application:ELISA, IHC, WB, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
HIF1a antibody
Catalogue No.
FNab10340
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Alternative Names
BHLHE78 antibody, MOP1 antibody, PASD8 antibody
UniProt ID
Q16665
Observed MW
120 kDa
Application
Tested Applications
ELISA, IHC, WB, IF
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Validated Images
Various lyastes were subjected to SDS PAGE followed by western blot with FNab10340(HIF1a Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney using FNab10340(HIF1A antibody) at dilution of 1:100
Background
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.