Products
Caspase 3 antibody
- Product Name
- Caspase 3 antibody
- Catalogue No.
- FNab10013
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- caspase 3, apoptosis-related cysteine peptidase
- Alternative Names
- Apopain antibody, CASP 3 antibody, CASP3 antibody, Caspase 3 antibody, Caspase 3/p17 antibody, Caspase3 antibody, CPP 32 antibody, CPP32 antibody, CPP32B antibody, Cysteine protease CPP32 antibody, Protein Yama antibody, SCA 1 antibody, SREBP cleavage activity 1 antibody
- UniProt ID
- P42574
- Observed MW
- 32-35 kDa, 17 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab10013(CASP3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using FNab10013(CASP3 Antibody) at dilution of 1:100
Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using FNab10013 (caspase 3 antibody) at dilution of 1:50 and FITC-Conjugated Goat Anti-rabbit IgG(H+L)
- Background
- Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.
