Products

anti- Caspase 3 antibody

FNab10013 Catalogue No.
Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- Caspase 3 antibody
Catalogue No.
FNab10013
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.
Immunogen Information
Immunogen
caspase 3, apoptosis-related cysteine peptidase
Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Observed MW
32-35 kDa, 17 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:200-1:2000; IHC: 1:50-1:500
Uniprot information
UniProt ID
P42574
Images
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using FNab10013(CASP3 Antibody) at dilution of 1:100
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab10013(CASP3 antibody) at dilution of 1:1000