Products
p65/RELA antibody
- Product Name
- p65/RELA antibody
- Catalogue No.
- FNab06089
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- v-rel reticuloendotheliosis viral oncogene homolog A(avian)
- Alternative Names
- NFKB 3 antibody, NFKB p65 antibody, NFKB3 antibody, p65 antibody, RELA antibody, Transcription factor p65 antibody
- UniProt ID
- Q04206
- Observed MW
- 65 kDa
Application
- Tested Applications
- ELISA, IHC, IF, IP, WB, FC
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab06089(p65 antibody) at dilution of 1:1000
IP Result of anti-p65 (IP:FNab06089, 3ug; Detection:FNab06089 1:1000) with HeLa cells lysate 5000ug.
Immunohistochemistry of paraffin-embedded human liver cancer using FNab06089(p65 antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FNab06089(RELA antibody)at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
