Products
TNF-alpha antibody
- Product Name
- TNF-alpha antibody
- Catalogue No.
- FNab08821
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tumor necrosis factor(TNF superfamily, member 2)
- Alternative Names
- Cachectin antibody, DIF antibody, TNF antibody, TNF a antibody, TNF alpha antibody, TNFA antibody, TNFSF2 antibody, Tumor necrosis factor antibody
- UniProt ID
- P01375
- Observed MW
- 26KD
Application
- Tested Applications
- ELISA, IHC,WB
- Recommended dilution
- WB: 1:200-1:1000; IHC: 1:50-1:500
Validated Images
mouse colon tissue were subjected to SDS PAGE followed by western blot with FNab08821(TNFA antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08821(TNF-a Antibody) at dilution of 1:50
- Background
- TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme(TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
