Products
Home » Products » Antibody » Primary Antibody » Cyclin B1 antibody
Cyclin B1 antibody
- SPECIFICATIONS
- CITATIONS
- Product Name
- Cyclin B1 antibody
- Catalogue No.
- FNab02122
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- cyclin B1
- Alternative Names
- CCNB antibody, CCNB1 antibody, cyclin B1 antibody, G2/mitotic specific cyclin B1 antibody
- UniProt ID
- P14635
- Observed MW
- 55 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02122(CCNB1 antibody) at dilution of 1:500
IP Result of anti-Cyclin B1 (IP:FNab02122, 4ug; Detection:FNab02122 1:500) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab02122(Cyclin B1 Antibody) at dilution of 1:50. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using FNab02122( Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor(MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1.
IF: 5.955
- Journal:
- Aging
- Author:
- Chongqing Medical and Pharmaceutical College, Chongqing 400030, China
- Cited Date:
- 2023-12-08
- Product: