Products

Cyclin B1 antibody

Synonyms:CCNB antibody, CCNB1 antibody, cyclin B1 antibody, G2/mitotic specific cyclin B1 antibody
Catalogue No.:FNab02122Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IF, IHC, IP
Clonality:polyclonalIsotype:IgG
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Product Name
Cyclin B1 antibody
Catalogue No.
FNab02122
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
cyclin B1
Alternative Names
CCNB antibody, CCNB1 antibody, cyclin B1 antibody, G2/mitotic specific cyclin B1 antibody
UniProt ID
P14635
Observed MW
55 kDa
Application
Tested Applications
ELISA, WB, IF, IHC, IP
Recommended dilution
WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02122(CCNB1 antibody) at dilution of 1:500
IP Result of anti-Cyclin B1 (IP:FNab02122, 4ug; Detection:FNab02122 1:500) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab02122(Cyclin B1 Antibody) at dilution of 1:50. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using FNab02122( Cyclin B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor(MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1.
IF: 5.955
Journal:
Aging
Author:
Chongqing Medical and Pharmaceutical College, Chongqing 400030, China
Cited Date:
2023-12-08
Product: