Products

Mouse NF-κB p65 (Nuclear Factor-κB p65) ELISA Kit

EM1231 Catalogue No.
NF-κB p65 ELISA Kit, NFkB p65 ELISA Kit, NFKB3 ELISA Kit, p65RelA ELISA Kit, MGC131774 ELISA Kit, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 ELISA Kit, p65 ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 ELISA Kit
Reactivity
Mouse
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Mouse NF-κB p65 (Nuclear Factor-κB p65) ELISA Kit
Catalogue No.
EM1231
Alias
NF-κB p65 ELISA Kit, NFkB p65 ELISA Kit, NFKB3 ELISA Kit, p65RelA ELISA Kit, MGC131774 ELISA Kit, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 ELISA Kit, p65 ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NF-κB p65 ELISA Kit allows for the in vitro quantitative determination of NF-κB p65 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9794
EDTA plasma(n=5)89-10095
heparin plasma(n=5)85-10294
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-105%95-102%85-97%89-105%
EDTA plasma(n=5)82-101%87-101%86-101%88-98%
heparin plasma(n=5)83-90%84-92%80-95%87-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only