- Product Name
- Mouse NF-κB p65 (Nuclear Factor-κB p65) ELISA Kit
- Catalogue No.
- EM1231
- Alias
- NF-κB p65 ELISA Kit, NFkB p65 ELISA Kit, NFKB3 ELISA Kit, p65RelA ELISA Kit, MGC131774 ELISA Kit, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 ELISA Kit, p65 ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- NF-κB p65 ELISA Kit allows for the in vitro quantitative determination of NF-κB p65 concentrations in plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Mouse
- Standard curve

- Storage
- 4 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 88-97 | 94 |
EDTA plasma(n=5) | 89-100 | 95 |
heparin plasma(n=5) | 85-102 | 94 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-105% | 95-102% | 85-97% | 89-105% |
EDTA plasma(n=5) | 82-101% | 87-101% | 86-101% | 88-98% |
heparin plasma(n=5) | 83-90% | 84-92% | 80-95% | 87-100% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only