Products

Bovine TNF-a(Tumor necrosis factor alpha)ELISA Kit

EB0008 Catalogue No.
TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Reactivity
Bovine
Range
4.69-300pg/ml
Sensitivity
2.813pg/ml
Product Name
Bovine TNF-a(Tumor necrosis factor alpha)ELISA Kit
Catalogue No.
EB0008
Alias
TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-aELISA Kit allows for the in vitro quantitative determination of TNF-Α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.69-300pg/ml
Sensitivity
< 2.813pg/ml
Species
Bovine
UniProt ID
Q06599
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNF-Α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-Α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10193
EDTA plasma(n=5)90-10596
heparin plasma(n=5)85-10597
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-Α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-102%85-100%88-105%87-97%
EDTA plasma(n=5)82-94%86-95%84-101%89-99%
heparin plasma(n=5)84-94%83-98%83-90%85-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only