Products
Nestin antibody
- Product Name
- Nestin antibody
- Catalogue No.
- FNab10534
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- nestin
- Alternative Names
- NES antibody, Nestin antibody
- UniProt ID
- P48681
- Observed MW
- 170 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab10534(NES antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain using FNab10534(NES antibody) at dilution of 1:50
Immunofluorescent analysis of SH-SY5Y cells using FNab10534(NES antibody) at dilution of 1:50.
- Background
- Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
