Products
Caspase 8 antibody
- Product Name
- Caspase 8 antibody
- Catalogue No.
- FNab01294
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 2C2
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- caspase 8, apoptosis-related cysteine peptidase
- Alternative Names
- Apoptotic cysteine protease antibody, Apoptotic protease Mch 5 antibody, Casp 8 antibody, CASP8 antibody, Caspase 8 antibody, Caspase 8/p18 antibody, Caspase8 antibody, FADD like ICE antibody, FLICE antibody, ICE like apoptotic protease 5 antibody, MACH antibody, MCH5 antibody
- UniProt ID
- Q14790
- Observed MW
- 50-55 kDa, 25-32 kDa
Application
- Tested Applications
- ELISA, IHC, WB,IF,IP
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:100-1:400; IF: 1:50-1:500
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab01294(CASP8 antibody) at dilution of 1:1000
IP Result of anti-Caspase 8 (IP:FNab01294, 5ug; Detection:FNab01294 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab01294(Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab01294 ( Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro-and cleaved-caspase 8.
