Products
RAB5A antibody
- Product Name
- RAB5A antibody
- Catalogue No.
- FNab07038
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- RAB5A, member RAS oncogene family
- Alternative Names
- RAB5 antibody, RAB5A antibody, Ras related protein Rab 5A antibody
- UniProt ID
- P20339
- Observed MW
- 24 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IP,IF
- Recommended dilution
- WB: 1:200-1:2000; IP:1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab07038(RAB5A antibody) at dilution of 1:500
IP Result of anti-RAB5A (IP:FNab07038, 4ug; Detection:FNab07038 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using FNab07038(RAB5A Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab07038( RAB5A Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes(PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension(PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan(PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3(By similarity).
