Products
ATG12 antibody
- Product Name
- ATG12 antibody
- Catalogue No.
- FNab00666
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ATG12 autophagy related 12 homolog
- Alternative Names
- APG12 antibody, APG12L antibody
- UniProt ID
- O94817
- Observed MW
- 48-50kd
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200
Validated Images
human bladder tissue were subjected to SDS PAGE followed by western blot with FNab00666(ATG12 antibody) at dilution of 1:500
IP Result of anti-ATG12 (IP:FNab00666, 4ug; Detection:FNab00666 1:300) with HEK-293 cells lysate 880ug.
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab00666(ATG12 antibody) at dilution of 1:50
- Background
- Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.
