FineTest Protein Service
FineTest® protein service has supplied nearly over 4,500 proteins expressed by HEK293 and E.Coli for WB, Elisa application. Besides His tag and GST tag, we developed special IF2DI tag with high-efficient solubility which can greatly improve the protein expression.
- Over 95% Purity
- Image-based Validation
- High Throughput Protein Expression and Purification
- Reproducible Protein Test
- Advanced Production Equipment
- Competitive Protein Price
IF2D1 Tag
FineTest develops pET28a-IF2D1 expression vector with the powerful fusion tag, which perfectly replaces GST, MBP etc. Furthermore, the molecular weight is lower and the influence on the expression of target proteins is less. Thrombin functions as the enzyme cutting site to facilitate the subsequent cutting.
Custom Recombinant Protein
FineTest can produce any custom protein according to customers' requirements. Only protein name, Uniprot ID and applications are required.
| Service Items | Service Checklist | Service Timeline |
| Gene Synthesis | Construction of Expression Plasmid | 1-2 weeks |
| Protein Expression | Transformation Competent Cells | Within 1 week |
| Protein Purification | Column Chromatography Purification or Refolding | 1 week |
| Project Delivery | Refolding, Lyophilized Protein, SDS-PAGE Result | 2-3 weeks |
REFERENCES
Currently, some proteins have been cited by international well-known journals, like Theranostics, Antioxidants etc. Some high IF citations are listed below:
- Article Title: Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction
IF: 11.556 Journal Title: Theranostics Target: Human Thymosin beta 4 - Article Title: Cyanidin-3-O-glucoside Regulates the Expression of Ucp1 in Brown Adipose Tissue by Activating Prdm16 Gene
IF: 6.313 Journal Title: Antioxidants Target: Human PPARA - Article Title: A hemicyanine-based fluorescent probe for simultaneous imaging of Carboxylesterases and Histone deacetylases in hepatocellular carcinoma
IF: 4.831 Journal Title: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Target: Human CES1/CES2