Custom Protein Development

FineTest Protein Service

FineTest® protein service has supplied nearly over 4,500 proteins expressed by HEK293 and E.Coli for WB, Elisa application. Besides His tag and GST tag, we developed special IF2DI tag with high-efficient solubility which can greatly improve the protein expression.

  • Over 95% Purity
  • Image-based Validation
  • High Throughput Protein Expression and Purification
  • Reproducible Protein Test
  • Advanced Production Equipment
  • Competitive Protein Price
P0142 P0142 Recombinant Human PAI-1 in SDS Page
P0171 P0171 Recombinant Rat IL-1b in SDS Page and Elisa

IF2D1 Tag

FineTest develops pET28a-IF2D1 expression vector with the powerful fusion tag, which perfectly replaces GST, MBP etc. Furthermore, the molecular weight is lower and the influence on the expression of target proteins is less. Thrombin functions as the enzyme cutting site to facilitate the subsequent cutting.

IF2D1 Tag

Custom Recombinant Protein

FineTest can produce any custom protein according to customers' requirements. Only protein name, Uniprot ID and applications are required.

custom recombinant protein service

Service Items Service Checklist Service Timeline
Gene Synthesis Construction of Expression Plasmid 1-2 weeks
Protein Expression Transformation Competent Cells Within 1 week
Protein Purification Column Chromatography Purification or Refolding 1 week
Project Delivery Refolding, Lyophilized Protein, SDS-PAGE Result 2-3 weeks


Currently, some proteins have been cited by international well-known journals, like Theranostics, Antioxidants etc. Some high IF citations are listed below: