Human MMP14(Matrix metalloproteinase-14) ELISA Kit

Manual
  • Specifications
Product name

Human MMP14(Matrix metalloproteinase-14) ELISA Kit

Catalogue No.

EH0369

Alias

MMP14 ELISA Kit, MMP-14 ELISA Kit, MMP-X1 ELISA Kit, MT1-MMP ELISA Kit, MT-MMP1 ELISA Kit, matrix metallopeptidase 14(membrane-inserted) ELISA Kit, membrane type 1 metalloprotease ELISA Kit, Membrane-type matrix metalloproteinase 1 ELISA Kit, Membrane-type-1 matrix metalloproteinase ELISA Kit, MT1MMP ELISA Kit, MTMMP1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP14 ELISA Kit allows for the in vitro quantitative determination of MMP14 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human MMP14(Matrix metalloproteinase-14) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP14 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP14 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 95
EDTA plasma(n=5) 86-102 93
heparin plasma(n=5) 89-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 89-105% 86-104% 86-105%
EDTA plasma(n=5) 82-100% 89-100% 82-101% 82-93%
heparin plasma(n=5) 87-100% 80-91% 82-98% 80-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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