3 minutes to know 2019-nCoV Detection Principle

Structure of 2019-nCoV

2019 novel coronavirus is also called 2019-nCoV, SARS-CoV-2, SARS-CoV2. Novel coronavirus and SARS coronavirus (SARS-CoV) are of the same class, but not the same species, with single-stranded RNA genes.

4 types of main Structure protein:

Spike Surface Glycoprotein (S)
Envelope Protein (E)
Matrix Protein (M)
Nucleocapsid Protein (N)

2019-nCoV related protein

S pike protein is big and it consists of 2 subunits, S1 and S2. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. Recent research mainly focuses on S1-RBD protein, which is target point for antibody drugs.

N protein and viral genome RNA entwined each other to form viral nucleocapsid, which plays an important role in the synthesis of viral RNA. At the same time, N protein is relatively conservative, which accounts for the largest proportion in the Structure protein of the virus. At the early stage of infection, the organism can produce high-level antibodies against N protein. Therefore, N protein can be used to establish a rapid 2019-ncov serum antibody detection method.

Latest Launched FineTest N protein Related Products

2019-nCov Nucleic Acid Testing Kit

Detection Principle
Qualitative detecting of both ORF1ab and N gene of 2019-nCoV by fluorescent PCR.

N protein gene is relatively conservative, and will cross with other virus genes, which may easily lead to misdiagnosis. While ORF1ab gene has high specificity, detecting both N protein and ORF1ab gene can be more effectively and could avoid misdiagnosis.
Nucleic Acid Detection takes an average of 2-3 hours, and the experiment process requires automatic nucleic acid extraction instrument and fluorescence quantitative PCR instrument.

2019-nCoV Rapid Detection Strips

Detection Principle
Using colloidal gold immunochromatography detects both IgG and IgM antibodies in (finger blood, serum, plasma) samples.

Significance
IgM + IgG joint detection-monitoring of 2019-nCoV infections throughout the cycle (early, middle and late)
• IgM and IgG are two different antibodies produced by the human body against pathogenic microorganisms (including viruses).
• IgM is the first antibody that appears after infection. The 2019-nCoV infection peaks after 3-7 days, and gradually decreases thereafter. IgM Detection can indicate early infection.
• IgG is an antibody produced in large quantities when B cells enter the lymph nodes and differentiate into plasma cells in the middle and late stage of infection. IgG detection indicates the recovery after infection or infection is taking place.