Products
DAB Substrate Kit
- Catalogue No.:
- IHC0005
- Size:
- 1200 sections
- Storage:
- Store at 2-8℃ for one year except for Reagents A at -20℃, away from light.
- Application:
- IHC
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- Catalogue No.
IHC0005
- Size
1200 tests
- Storage
Store at 2-8℃ for one year except for Reagents A at -20℃, away from light.
- Background
FineTest DAB Substrate (3, 3’-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. Substrate Kit contains all of the reagents necessary toprepare.
- REAGENTS
Reagents A
DAB 20×concentrated solution
3ml
-20℃
Reagents B
H2O2 20×concentrated solution
3ml
2-8℃
TBS 20× concentrated solution
3ml
2-8℃
Avoid storing reagents or working solution in strong direct light
- PREPARATION OF SUBSTRATE WORKING SOLUTION
- To 1.0 ml of distilled water
Add 50 µl of Reagents C
Add 50 µl of Reagents A
Add 50 µl of Reagents B
- Mix well before use
- INSTRUCTIONS FOR USE
For Tissues or Cells
After incubation with a peroxidase (HRP) detection system, rinse well. Incubate with the substrate working solution at room temperature for 2-10 minutes. Optimal development times should be determined by the investigator.
Wash for 5 minutes in water.
- NOTES
- We recommend using glass-distilled water in the preparation of substrate buffer. Deionized water may con-tain inhibitors of the peroxidase reaction. Solutions containing sodium azide or other inhibitors of peroxidase activity should not be used in diluting the peroxidase substrate.
- DAB are carcinogens. Appropriate care should be exercised when using these reagents including gloves, eye protection, lab coats, and good laboratory procedures.
For Laboratory Use!
- Journal:
- Cellular Oncology
- Author:
- Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China
- Cited Date:
- 2023-09-22
- Product:
- Journal:
- Neuropharmacology
- Author:
- Laboratory of Neurophysiology of Pain, Department of Physiology, Division of Biological Sciences, Federal University of Paraná, Curitiba, Paraná, Brazil.
- Cited Date:
- 2024-07-05
- Product:
- Journal:
- Bioengineered
- Cited Date:
- 2022-01-14
- Product: