DAB Substrate Kit

Catalogue No.: 
IHC0005
Size: 
1200 sections
Storage: 
Store at 2-8℃ for one year except for Reagents A at -20℃, away from light.
Application: 
IHC
  • SPECIFICATIONS
  • CITATIONS
  • FIGURES
  • CONDITIONS
Catalogue No.

IHC0005

Size

1200 tests

Storage

Store at 2-8℃ for one year except for Reagents A at -20℃, away from light.

Background

FineTest DAB Substrate (3, 3’-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. Substrate Kit contains all of the reagents necessary toprepare.

REAGENTS

Reagents A

DAB 20×concentrated solution

3ml

-20℃

Reagents B

H2O2 20×concentrated solution

3ml

2-8℃

Reagents C

TBS 20× concentrated solution

3ml

2-8℃

Avoid storing reagents or working solution in strong direct light

PREPARATION OF SUBSTRATE WORKING SOLUTION
  • To 1.0 ml of distilled water
  • Add 50 µl of Reagents C

    Add 50 µl of Reagents A

    Add 50 µl of Reagents B

  • Mix well before use
  • INSTRUCTIONS FOR USE

    For Tissues or Cells

    After incubation with a peroxidase (HRP) detection system, rinse well. Incubate with the substrate working solution at room temperature for 2-10 minutes. Optimal development times should be determined by the investigator.

    Wash for 5 minutes in water.

    NOTES
  • We recommend using glass-distilled water in the preparation of substrate buffer. Deionized water may con-tain inhibitors of the peroxidase reaction. Solutions containing sodium azide or other inhibitors of peroxidase activity should not be used in diluting the peroxidase substrate.
  • DAB are carcinogens. Appropriate care should be exercised when using these reagents including gloves, eye protection, lab coats, and good laboratory procedures.
  • For Laboratory Use!

    IF: 6.6
    Journal:
    Cellular Oncology
    Author:
    Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China
    Cited Date:
    2023-09-22
    Product:
    IF: 4.6
    Journal:
    Neuropharmacology
    Author:
    Laboratory of Neurophysiology of Pain, Department of Physiology, Division of Biological Sciences, Federal University of Paraná, Curitiba, Paraná, Brazil.
    Cited Date:
    2024-07-05
    Product: