Products

DAB Substrate Kit

IHC0005 Catalogue No.
Overview
Catalogue No.
IHC0005

FineTest DAB Substrate (3, 3’-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. Substrate Kit contains all of the reagents necessary toprepare. These reagents are supplied in convenient dropper bottles.

REAGENTS
ContentSizeStorage
Reagents ADAB 20×concentrated solution3ml-20℃
Reagents BH2O2 20×concentrated solution3ml4-8℃
Reagents CTBS 20× concentrated solution3ml4-8℃
STORAGE
  • Store reagents in original bottles at the required conditions (one year)
  • Avoid storing reagents or working solution in strong direct light.


PREPARATION OF SUBSTRATE WORKING SOLUTION
  • To 1.0 ml of distilled water
    • Add 50 µl of Reagents C
    • Add 50 µl of Reagents A
    • Add 50 µl of Reagents B
  • Mix well before use
INSTRUCTIONS FOR USE

For Tissues or Cells After incubation with a peroxidase (HRP) detection system, rinse well. Incubate with the substrate working solution at room temperature for 2-10 minutes. Optimal development times should be determined by the investigator. Wash for 5 minutes in water.

NOTES
  • We recommend using glass-distilled water in the preparation of substrate buffer. Deionized water may con-tain inhibitors of the peroxidase reaction. Solutions containing sodium azide or other inhibitors of peroxidase activity should not be used in diluting the peroxidase substrate.
  • DAB are carcinogens. Appropriate care should be exercised when using these reagents including gloves, eye protection, lab coats, and good laboratory procedures.