Products
HRP-Goat Anti-Rabbit IgG (H+L)
| Size | Price |
|---|---|
| 100μl | Inquiry |
| 500μl | Inquiry |
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- HRP-Goat Anti-Rabbit IgG (H+L)
- Catalogue No.
- FNSA-0004
- Size
- 100μl/500μl
- Form
- liquid
- Conjugation
- HRP
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20℃ for 24 months , avoid repeated freezing and thawing.
- Tested Applications
- ELISA, WB, IHC
- Suggested dilution
- 1:1000-1:10000 for western blotting with ECL substrates1:1000-1:20000 for ELISA and Western blotting with chromogenic substrates1:20-1:50 for IHC
- Journal:
- Nature Communications
- Author:
- State Key Laboratory of Rare Earth Resource Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P. R. China
- Cited Date:
- 2023-04-14
- Product:
- Journal:
- Brain, Behavior, and Immunity
- Author:
- School of Clinical Medicine, Chengdu Medical College, Chengdu 610500, Sichuan Province, China; Department of Anesthesiology, Sichuan Provincial Maternity and Child Health Care Hospital, Chengdu 610500, Sichuan Province, China.
- Cited Date:
- 2024-09-27
- Product:
- Journal:
- Biomaterials Advances
- Author:
- Department of Bone & Joint Surgery, National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, China
- Cited Date:
- 2024-05-24
- Product:
- Journal:
- Industrial Crops and Products
- Author:
- Department of Traditional Chinese Medicine, Institute of Guangdong Geriatric, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China
- Cited Date:
- 2023-04-28
- Product:
- Journal:
- Cellular Signalling
- Author:
- Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, China.
- Cited Date:
- 2024-12-20
- Product:
- Journal:
- Scientific Reports
- Author:
- Department of Paediatrics, Affiliated Hospital of North Sichuan Medical College, No. 1 Maoyuan South Road, Shunqing District, Nanchong, 637000, Sichuan, China.
- Sample:
- SK-N-SH cells
- Cited Date:
- 2024-09-06
- Product:
- Journal:
- Cell Biochemistry and Biophysics
- Author:
- Department of Traditional Chinese Medicine, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China.
- Cited Date:
- 2024-05-31
- Product:
- Journal:
- Current Issues in Molecular Biology
- Cited Date:
- 2021-12-17
- Product:
- Journal:
- Research Square
- Cited Date:
- 2022-09-16
- Product:
- Journal:
- IOP Conference Series: Earth and Environmental Science
- Author:
- Department of Biology, College of Education for Pure Science, Mosul University, Mosul, Iraq
- Cited Date:
- 2025-04-04
- Product:
- Journal:
- Journal of Applied Veterinary Sciences
- Author:
- Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Tikrit, Iraq
- Sample:
- bone tissues
- Cited Date:
- 2024-03-15
- Product:
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).