Products
PTGS2 antibody
| Synonyms： | Prostaglandin G/H synthase 2|Cyclooxygenase-2 (COX-2)|PHS II|Prostaglandin H2 synthase 2 (PGH synthase 2 antibody, PGHS-2)|Prostaglandin-endoperoxide synthase 2|PTGS2|COX2 antibody | ||
| Catalogue No.： | FNab10407 | Reactivity： | Human, Mouse, Rat |
| Host： | Rabbit | Tested Application： | ELISA, WB, IHC |
| Clonality： | polyclonal | Isotype： | IgG |
| Size | Price |
|---|---|
| 100ug | Inquiry |
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- PTGS2 antibody
- Catalogue No.
- FNab10407
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Immunogen
- prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase)
- Alternative Names
- Prostaglandin G/H synthase 2|Cyclooxygenase-2 (COX-2)|PHS II|Prostaglandin H2 synthase 2 (PGH synthase 2 antibody, PGHS-2)|Prostaglandin-endoperoxide synthase 2|PTGS2|COX2 antibody
- UniProt ID
- P35354
- Observed MW
- 50 kDa, 80 kDa
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Various lysates were subjected to SDS PAGE followed by western blot with FNab10407(PTGS2 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human liver using FNab10407(cox2 antibody) at dilution of 1:100
- Background
- COX2 (Prostaglandin G/H synthase 2; PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72–74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).
- Journal:
- Chemical Engineering Journal
- Author:
- National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, Guangdong, PR China
- Cited Date:
- 2024-09-20
- Product:
- Journal:
- Molecular Neurobiology
- Author:
- Molecular Toxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, 110062, India
- Cited Date:
- 2024-01-05
- Product:
- Journal:
- iScience
- Author:
- National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, People's Republic of China.
- Sample:
- chondrocytes
- Cited Date:
- 2024-09-06
- Product:
- Journal:
- American Journal of Translational Research
- Cited Date:
- 2022-02-11
- Product:
- Journal:
- Biomedicines
- Author:
- National & Local Joint Engineering Research Centre of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen 518036, China
- Sample:
- cartilage samples
- Cited Date:
- 2024-08-23
- Product:
- Journal:
- Food Research
- Cited Date:
- 2022-05-06
- Product:
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).