Products

anti- PTEN antibody

FNab10258 Catalogue No.
Synonyms
MMAC1, TEP1
Host
Rabbit
Reactivity
Human, Rat
Isotype
IgG
Tested Application
ELISA, WB
Product Information
Product Name
anti- PTEN antibody
Catalogue No.
FNab10258
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Background
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
phosphatase and tensin homolog
Synonyms
MMAC1, TEP1
Observed MW
55 kDa
Application
Tested Application
ELISA, WB
Recommended dilution
WB: 1:500 - 1:2000
Uniprot information
UniProt ID
P60484
Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab10258( PTEN Antibody) at dilution of 1:1000