Jurkat cells were subjected to SDS PAGE followed by western blot with FNab10125( TIM3 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab10125 (TIM3 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Background
TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected probably represent glycosylated TIM3.