Products

anti- HIF1a antibody

FNab09940 Catalogue No.
Synonyms
BHLHE78, MOP1, PASD8
Host
Mouse
Reactivity
Human
Isotype
IgG1
Tested Application
ELISA, IF, WB
Product Information
Product Name
anti- HIF1a antibody
Catalogue No.
FNab09940
Size
100µg
Form
liquid
Purification
protein A+G purified
Purity
≥95% as determined by SDS-PAGE
Host
Mouse
Clonality
monoclonal
Isotype
IgG1
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
Immunogen Information
Immunogen
hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Synonyms
BHLHE78, MOP1, PASD8
Observed MW
120 kDa
Application
Tested Application
ELISA, IF, WB
Recommended dilution
WB: 1:1000-1:10000; IF: 1:50-1:200
Uniprot information
UniProt ID
Q16665
Images
Immunofluorescent analysis of ( -20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using FNab09940 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L)
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab09940(HIF1a Antibody) at dilution of 1:5000