Products
Asprosin antibody
| Size | Price |
|---|---|
| 100ug | Inquiry |
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- Asprosin antibody
- Catalogue No.
- FNab09797
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Immunogen
- asprosin
- Alternative Names
- Fibrillin-1|Asprosin|FBN1|FBN antibody
- UniProt ID
- P35555
- Observed MW
- 20-30 kDa
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:200-1:500
recombinant proteins were subjected to SDS PAGE followed by western blot with FNab09797(asprosin Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab09797(asprosin Antibody) at dilution of 1:400
- Background
- Hormone that targets the liver to increase plasma glucose levels. Secreted by white adipose tissue and circulates in the plasma. Acts in response to fasting and promotes blood glucose elevation by binding to the surface of hepatocytes. Promotes hepatocyte glucose release by activating the protein kinase A activity in the liver, resulting in rapid glucose release into the circulation.
- Journal:
- Journal of Translational Medicine
- Cited Date:
- 2022-08-05
- Product:
- Journal:
- American Journal of Biomedical Sciences
- Cited Date:
- 2019-09-12
- Product:
- Journal:
- Molecular Nutrition & Food Research
- Cited Date:
- 2021-12-31
- Product:
- Journal:
- International Journal of Molecular Sciences
- Author:
- Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China
- Cited Date:
- 2023-12-01
- Product:
- Journal:
- Scientific Reports
- Author:
- Department of Exercise Physiology, Faculty of Sport Sciences, University of Guilan, Rasht, Iran.
- Species:
- supernatants
- Cited Date:
- 2024-06-14
- Product:
- Journal:
- Heliyon
- Author:
- Department of Medical Pharmacology, Faculty of Medicine, Adiyaman University, Adiyaman, 02040, Turkey
- Cited Date:
- 2023-08-04
- Product:
- Journal:
- Molecular and Cellular Endocrinology
- Cited Date:
- 2019-03-07
- Product:
- Journal:
- Pathology - Research and Practice
- Cited Date:
- 2023-04-14
- Product:
- Journal:
- Immunity, Inflammation and Disease
- Author:
- Department of Infectious Disease, The Affiliated People's Hospital of Ningbo University, Ningbo City, Zhejiang Province, China
- Cited Date:
- 2023-08-25
- Product:
- Journal:
- Tissue and Cell
- Author:
- Firat University School of Medicine, Department of Histology and Embryology Elazig, Turkey
- Cited Date:
- 2024-06-28
- Product:
- Journal:
- Tissue and Cell
- Cited Date:
- 2022-02-11
- Product:
- Journal:
- Tissue and Cell
- Cited Date:
- 2022-06-24
- Product:
- Journal:
- Biotechnic & Histochemistry
- Cited Date:
- 2023-03-31
- Product:
- Journal:
- Biotechnic & Histochemistry
- Author:
- Department of Dermatology, Biruni University Faculty of Medicine, Istanbul, Turkey
- Cited Date:
- 2024-01-19
- Product:
- Journal:
- Biotechnic & Histochemistry
- Cited Date:
- 2019-10-01
- Product:
- Journal:
- Biotechnic & Histochemistry
- Cited Date:
- 2020-02-03
- Product:
- Journal:
- Medicine Science
- Author:
- Adıyaman University, Faculty of Medicine, Department of Histology and Embryology, Adıyaman, Türkiye
- Cited Date:
- 2023-10-13
- Product:
- Journal:
- Kafkas Universitesi Veteriner Fakultesi Dergisi
- Author:
- Ad?yaman University, Faculty of Medicine, Anesthesiology and Reanimation Department, TR-02100 Ad?yaman - T?RK?YE
- Cited Date:
- 2023-10-20
- Product:
- Journal:
- Cureus
- Author:
- Department of Histology and Embryology, Firat University Faculty of Medicine, Elaz??, TUR
- Sample:
- breast tissue samples
- Cited Date:
- 2024-07-12
- Product:
- Journal:
- F?rat University Journal of Health Sciences
- Cited Date:
- 2022-07-15
- Product:
- Journal:
- Cureus
- Author:
- Medical Pharmacology, Adiyaman University, Adiyaman, TUR
- Cited Date:
- 2023-11-03
- Product:
- Journal:
- Cureus
- Author:
- Department of Histology and Embryology, Faculty of Medicine, Adiyaman University, Adiyaman, TUR
- Cited Date:
- 2023-11-03
- Product:
- Journal:
- Chronicles of Precision Medical Researchers
- Author:
- Ad?yaman University
- Cited Date:
- 2023-11-10
- Product:
- Journal:
- Turkish Journal of Veterinary Research
- Cited Date:
- 2023-02-10
- Product:
- Journal:
- Archives of Medical Science - Civilization Diseases
- Author:
- Department of Histology and Embryology, Faculty of Medicine, F?rat University, Elaz??, Turkey
- Sample:
- heart tissues
- Cited Date:
- 2024-10-08
- Product:
- Journal:
- Van Medical Journal
- Author:
- Firat University Hospital, Faculty of Medicine, Department of Dermatology, Elazig, Türkiye
- Cited Date:
- 2025-01-24
- Product:
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).