Products
TAP1 antibody
- Product Name
- TAP1 antibody
- Catalogue No.
- FNab08499
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- transporter 1, ATP-binding cassette, sub-family B(MDR/TAP)
- Alternative Names
- ABCB2 antibody, PSF1 antibody, RING4 antibody, Y3 antibody
- UniProt ID
- Q03518
- Observed MW
- 70-81 kDa
Application
- Tested Applications
- ELISA, IF, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab08499(TAP1 Antibody) at dilution of 1:1000
IP Result of anti-TAP1 (IP:FNab08499, 4ug; Detection:FNab08499 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab08499(TAP1 Antibody) at dilution of 1:50
- Background
- Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
