Products
PKM1-specific antibody
- Product Name
- PKM1-specific antibody
- Catalogue No.
- FNab06494
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- PKM2 pyruvate kinase, muscle
- Alternative Names
- CTHBP antibody, OIP 3 antibody, OIP3 antibody, Opa interacting protein 3 antibody, p58 antibody, PK2 antibody, PK3 antibody, PKM antibody, PKM1 antibody, PK-M1 antibody, PKM1 pyruvate kinase antibody, muscle antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, TCB antibody, THBP1 antibody, Tumor M2 PK antibody
- UniProt ID
- P14618
- Observed MW
- 58KD
Application
- Tested Applications
- ELISA, WB, IHC, IP, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:100-1:400; IF: 1:20-1:200
Validated Images
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06494(PKM1 antibody) at dilution of 1:1000
IP Result of anti-PKM1-specific (IP:FNab06494, 3ug; Detection:FNab06494 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using FNab06494(PKM1 Antibody) at dilution of 1:200
Immunofluorescent analysis of HeLa cells using FNab06494 (PKM1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
- Background
- PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2(PMID:18337823). This antibody is specific to PKM1 isoform.
