Products
HSP70 antibody
- Product Name
- HSP70 antibody
- Catalogue No.
- FNab04048
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- heat shock 70kDa protein 1A
- Alternative Names
- Heat shock 70 kDa protein 1A antibody, HSPA1A antibody, Heat shock 70 kDa protein 1 antibody, HSP70-1 antibody, HSPA1 antibody, HSX70 antibody
- UniProt ID
- P0DMV8
- Observed MW
- 66-70 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IF, FC, IP
- Recommended dilution
- WB: 1:1000-1:10000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab04048(HSP70 Antibody) at dilution of 1:5000
IP Result of anti-HSP70 (IP: FNab04048, 3ug; Detection: FNab04048 1:1000) with mouse brain tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab04048(HSP70 Antibody) at dilution of 1:200
Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody FNab04048 at 1:50 dilution. Blue pseudocolor = DAPI (fluorescent DNA dye).
- Background
- HSPA1A, collectively known as HSP70(also referred to HSP72), is a stress-inducible member of heat-shock protein 70(HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines.
