mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab03937(HMOX1 antibody) at dilution of 1:600
IP Result of anti-HMOX1 (IP:FNab03937, 3ug; Detection:FNab03937 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab03937(HO-1 Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HT-1080 cells using FNab03937 (HO-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.