Products
GRP94 antibody
- Product Name
- GRP94 antibody
- Catalogue No.
- FNab03663
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- heat shock protein 90kDa beta(Grp94), member 1
- Alternative Names
- ECGP antibody, Endoplasmin antibody, GP96 antibody, gp96 homolog antibody, GRP 94 antibody, GRP94 antibody, HSP90B1 antibody, TRA1 antibody, Tumor rejection antigen 1 antibody
- UniProt ID
- P14625
- Observed MW
- 94 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC, IF, IP
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
A549 cells were subjected to SDS PAGE followed by western blot with FNab03663(HSP90B1 antibody) at dilution of 1:1000
IP Result of anti-GRP94 (IP: FNab03663, 3ug; Detection: FNab03663 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab03663(HSP90B1 antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody FNab03663 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1(GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors(TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.
