GLAST antibody

Synonyms:EA6 antibody, EAAT1 antibody, GLAST antibody, GLAST 1 antibody, GLAST1 antibody, SLC1A3 antibody
Catalogue No.:FNab03475Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, IHC, WB
Clonality:polyclonalIsotype:IgG
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Product Name
GLAST antibody
Catalogue No.
FNab03475
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
Excitatory amino acid transporter 1
Alternative Names
EA6 antibody, EAAT1 antibody, GLAST antibody, GLAST 1 antibody, GLAST1 antibody, SLC1A3 antibody
UniProt ID
P43003
Observed MW
50-55, 65-70 kDa, 90-100 kDa
Application
Tested Applications
ELISA, IHC, WB
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
Various lysates were subjected to SDS PAGE followed by western blot with FNab03475(GLAST Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab03475(GLAST Antibody) at dilution of 1:50
Background
SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate.Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3.