mouse kidney were subjected to SDS PAGE followed by western blot with Nab03330(GALT antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse spleen using FNab03330(GALT antibody) at dilution of 1:100
Background
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.