Products
beta-Catenin antibody
- Product Name
- beta-Catenin antibody
- Catalogue No.
- FNab00882
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- catenin(cadherin-associated protein), beta 1, 88kDa
- Alternative Names
- b cat antibody, Beta catenin antibody, beta-Catenin antibody, Catenin beta 1 antibody, CTNNB antibody, CTNNB1 antibody, PRO2286 antibody, β catenin antibody, β-catenin antibody
- UniProt ID
- P35222
- Observed MW
- 90 kDa
Application
- Tested Applications
- ELISA, IF, WB, IHC, IP
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:500-1:1000; IHC: 1:100-1:400; IF: 1:20-1:200
Validated Images
mouse lung tissue were subjected to SDS PAGE followed by western blot with FNab00882(b-catentin antibody) at dilution of 1:1000
IP Result of anti-beta-Catenin (IP: FNab00882, 4ug; Detection: FNab00882 1:500) with mouse liver tissue lysate 3200ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab00882(B-catenin Antibody) at dilution of 1:200
Immunofluorescent analysis of HepG2 cells, using FNab00882(B-catenin Antibody) at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Background
- The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
