Products

anti- 4EBP1 antibody

FNab00006 Catalogue No.
Synonyms
4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2b
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- 4EBP1 antibody
Catalogue No.
FNab00006
Size
100µg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Host
Mouse
Clonality
monoclonal
Isotype
IgG2b
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Background
Eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) , is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.
Immunogen Information
Immunogen
eukaryotic translation initiation factor 4E binding protein 1
Synonyms
4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I
Observed MW
12 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:1000-1:10000; IHC: 1:20-1:200; IF: 1:20-1:200
Uniprot information
UniProt ID
Q13541
Images
Immunohistochemistry of paraffin-embedded human pancreas slide using FNab00006 (4EBP1 Antibody) at dilution of 1:50
K-562 cells were subjected to SDS PAGE followed by western blot with FNab00006 (4EBP1 antibody) at dilution of 1:2000