Products
Biotin ELISA Kit
- SPECIFICATIONS
- CITATIONS
- Product Name
- Biotin ELISA Kit
- Alias
- Biotin ELISA Kit
- Catalogue No.
- EU2591
- Size
- 48T/96T
- Species
- Universal
- UniProt No.
- CAS: 58-85-5
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Competitive ELISA, Coated with Antigen
- Detection Wavelength
- OD450
- Reaction Duration
- 1 hours
- Range
- 100-2000pg/ml
- Sensitivity
- 10pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Biotin , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 2.23 2.343 2.275 100 1.901 1.998 1.94 200 1.452 1.526 1.482 300 1.185 1.245 1.209 400 0.997 1.048 1.017 500 0.755 0.793 0.77 1000 0.349 0.367 0.356 2000 0.147 0.155 0.15 - Recovery
-
Add a certain amount of Biotin into the sample. Calculate the recovery by comparing the measured value with the expected amount of Biotin in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 86-104 95 EDTA plasma(n=10) 90-98 93 Heparin plasma(n=10) 95-105 99 - Linearity
-
Dilute the sample with a certain amount of Biotin at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 88-102% 82-101% 80-96% EDTA plasma(n=10) 86-99% 82-97% 94-99% Heparin plasma(n=10) 87-103% 87-98% 85-98% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 261.8 449.1 987.2 262.1 451.4 989.4 Standard deviation 14.22 17.74 39.29 15.44 21.17 51.75 CV(%) 5.43 3.95 3.98 5.89 4.69 5.23 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
- Journal:
- Diagnostics
- Cited Date:
- 2022-07-28
- Product: