Products

Biotin ELISA Kit

Alias:Biotin ELISA Kit
Catalogue No.:EU2591Species:Universal
Range:100-2000pg/mlSensitivity:10pg/ml
  • SPECIFICATIONS
  • CITATIONS
Product Name
Biotin ELISA Kit
Alias
Biotin ELISA Kit
Catalogue No.
EU2591
Size
48T/96T
Species
Universal
UniProt No.
CAS: 58-85-5
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Competitive ELISA, Coated with Antigen
Detection Wavelength
OD450
Reaction Duration
1 hours
Range
100-2000pg/ml
Sensitivity
10pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with Biotin , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 2.23 2.343 2.275
100 1.901 1.998 1.94
200 1.452 1.526 1.482
300 1.185 1.245 1.209
400 0.997 1.048 1.017
500 0.755 0.793 0.77
1000 0.349 0.367 0.356
2000 0.147 0.155 0.15
Recovery

Add a certain amount of Biotin into the sample. Calculate the recovery by comparing the measured value with the expected amount of Biotin in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 86-104 95
EDTA plasma(n=10) 90-98 93
Heparin plasma(n=10) 95-105 99
Linearity

Dilute the sample with a certain amount of Biotin at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 88-102% 82-101% 80-96%
EDTA plasma(n=10) 86-99% 82-97% 94-99%
Heparin plasma(n=10) 87-103% 87-98% 85-98%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 261.8 449.1 987.2 262.1 451.4 989.4
Standard deviation 14.22 17.74 39.29 15.44 21.17 51.75
CV(%) 5.43 3.95 3.98 5.89 4.69 5.23
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100