Products

VB12 (Vitamin B12) ELISA Kit

EU2539 Catalogue No.
VB12 (Vitamin B12) ELISA Kit
Reactivity
Universal
Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Product Name
VB12 (Vitamin B12) ELISA Kit
Catalogue No.
EU2539
Alias
VB12 (Vitamin B12) ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
VB12 ELISA Kit allows for the in vitro quantitative determination of VB12 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Universal
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of VB12 and the recovery rates were calculated by comparing the measured value to the expected amount of VB12 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10593
EDTA plasma(n=5)88-10598
heparin plasma(n=5)86-10296
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VB12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-104%86-103%85-105%97-105%
EDTA plasma(n=5)86-99%82-93%82-95%83-98%
heparin plasma(n=5)80-99%82-98%80-100%91-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only