Products

Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

EM0310 Catalogue No.
Hif1α ELISA Kit, HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Reactivity
Mouse
Range
125-8000pg/ml
Sensitivity
75pg/ml
Product Name
Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Catalogue No.
EM0310
Alias
Hif1α ELISA Kit, HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hif1α ELISA Kit allows for the in vitro quantitative determination of Hif1α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
125-8000pg/ml
Sensitivity
< 75pg/ml
Species
Mouse
UniProt ID
Q61221
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of Hif1α and the recovery rates were calculated by comparing the measured value to the expected amount of Hif1α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10294
EDTA plasma(n=5)89-10597
heparin plasma(n=5)87-10394
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hif1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-104%85-102%85-99%86-102%
EDTA plasma(n=5)87-101%82-101%85-99%87-100%
heparin plasma(n=5)93-96%82-98%85-100%81-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only