- Product Name
- Human DHFR(Dihydrofolate reductase) ELISA Kit
- Catalogue No.
- EH2463
- Alias
- DHFR ELISA Kit, DHFR ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, DHFRP1 ELISA Kit, dihydrofolate reductase ELISA Kit, DYR ELISA Kit, EC 1.5.1.3 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- DHFR ELISA Kit allows for the in vitro quantitative determination of DHFR concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 78.125-5000pg/ml
- Sensitivity
- < 46.875pg/ml
- Species
- Human
- UniProt ID
- P00374
- Standard curve

- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of DHFR and the recovery rates were calculated by comparing the measured value to the expected amount of DHFR in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 87-105 | 97 |
EDTA plasma(n=5) | 94-102 | 97 |
heparin plasma(n=5) | 86-102 | 92 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHFR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-93% | 85-102% | 86-103% |
EDTA plasma(n=5) | 83-101% | 90-98% | 83-99% |
heparin plasma(n=5) | 80-95% | 85-99% | 84-93% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only