- Product Name
- Bovine IL1ra(Interleukin 1 Receptor Antagonist) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- IL1ra ELISA Kit allows for the in vitro quantitative determination of IL1ra concentrations in plasma, tissue homogenates and other biological fluids.
- < 18.75pg/ml
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of IL1ra and the recovery rates were calculated by comparing the measured value to the expected amount of IL1ra in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-101 95 EDTA plasma(n=5) 87-105 95 heparin plasma(n=5) 91-104 94
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1ra and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 87-99% 86-103% 87-102% 86-105% EDTA plasma(n=5) 88-100% 85-93% 84-95% 83-94% heparin plasma(n=5) 83-93% 80-97% 84-94% 81-95%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area