Products

Bovine IL1ra(Interleukin 1 Receptor Antagonist) ELISA Kit

EB0169 Catalogue No.
Reactivity
Bovine
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Bovine IL1ra(Interleukin 1 Receptor Antagonist) ELISA Kit
Catalogue No.
EB0169
Detection Method
Sandwich ELISA, Double Antibody
Application
 IL1ra ELISA Kit allows for the in vitro quantitative determination of IL1ra concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Bovine
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL1ra and the recovery rates were calculated by comparing the measured value to the expected amount of IL1ra in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10195
EDTA plasma(n=5)87-10595
heparin plasma(n=5)91-10494
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1ra and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-99%86-103%87-102%86-105%
EDTA plasma(n=5)88-100%85-93%84-95%83-94%
heparin plasma(n=5)83-93%80-97%84-94%81-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only