Products

Bovine E2 (Estradiol ) ELISA Kit

EB0081 Catalogue No.
E2(Estradiol) ELISA Kit
Reactivity
Bovine
Range
2.5-800pg/ml
Sensitivity
7.5pg/ml
Product Name
Bovine E2 (Estradiol ) ELISA Kit
Catalogue No.
EB0081
Alias
E2(Estradiol) ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
E2 ELISA Kit allows for the in vitro quantitative determination of E2 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
2.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Bovine
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10598
EDTA plasma(n=5)85-10593
heparin plasma(n=5)88-10196
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-105%96-104%85-100%87-99%
EDTA plasma(n=5)82-99%86-101%82-97%83-100%
heparin plasma(n=5)84-97%80-98%86-100%81-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only