Products
CHAF1b antibody
- Product Name
- CHAF1b antibody
- Catalogue No.
- FNab10505
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Chromatin assembly factor 1 subunit B
- Alternative Names
- CHAF1B antibody, CAF-1 antibody, CAF-IP60 antibody, CAF1 antibody
- UniProt ID
- Q13112
- Observed MW
- 70 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab10505(CHAF1b antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse spleen slide using FNab10505(CHAF1b antibody) at dilution of 1:50
Immunofluorescent analysis of MCF-7 cells using FNab10505(CHAF1b antibody) at dilution of 1:50.
- Background
- Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
