Products
ALPL antibody
- Product Name
- ALPL antibody
- Catalogue No.
- FNab10377
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- alkaline phosphatase, liver/bone/kidney
- Alternative Names
- Alkaline phosphatase antibody, Alkaline phosphatase liver/bone/kidney isozyme antibody, Alkaline phosphatase tissue nonspecific isozyme antibody, ALPL antibody, BLAP antibody, Bone Alkaline Phosphatase antibody, NBAP antibody
- UniProt ID
- P05186
- Observed MW
- 45-60 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab10377(ALPL antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human kidney using FNab10377(ALPL antibody) at dilution of 1:50
- Background
- There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney(tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H(Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol(GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.
