Products
Cytochrome c antibody
- Product Name
- Cytochrome c antibody
- Catalogue No.
- FNab098271
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 3G4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Cytochrome c
- Alternative Names
- CYC antibody, CYCS antibody, Cytochrome c antibody, cytochrome c antibody, somatic antibody, HCS antibody, THC4 antibody
- UniProt ID
- P99999
- Observed MW
- 12 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:5000-1:30000; IHC: 1:100-1:1000
Validated Images
Hela cells were subjected to SDS PAGE followed by western blot with FNab098271(Cytochrome c antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab098271( Cytochrome c Antibody) at dilution of 1:600
- Background
- Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
