Vimentin antibody

Synonyms:FLJ36605 antibody, VIM antibody, Vimentin antibody
Catalogue No.:FNab09408Reactivity:Human
Host:RabbitTested Application:ELISA, WB, IHC, IF
Clonality:polyclonalIsotype:IgG
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Product Name
Vimentin antibody
Catalogue No.
FNab09408
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
vimentin
Alternative Names
FLJ36605 antibody, VIM antibody, Vimentin antibody
UniProt ID
P08670
Observed MW
55 kDa
Application
Tested Applications
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab09408(VIM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis using FNab09408(VIM antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FNab09408(VIM antibody) at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Background
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.