Products
UBE2F antibody
- Product Name
- UBE2F antibody
- Catalogue No.
- FNab09173
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ubiquitin-conjugating enzyme E2F(putative)
- Alternative Names
- NCE2 antibody, NEDD8 carrier protein UBE2F antibody, NEDD8 conjugating enzyme 2 antibody, NEDD8 protein ligase UBE2F antibody, UBE2F antibody
- UniProt ID
- Q969M7
- Observed MW
- 21 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09173(UBE2F antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human skin using FNab09173(UBE2F antibody) at dilution of 1:100
- Background
- Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
