Products
TRAPPC3 antibody
- Product Name
- TRAPPC3 antibody
- Catalogue No.
- FNab08947
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- trafficking protein particle complex 3
- Alternative Names
- BET3 antibody, BET3 homolog antibody, TRAPPC3 antibody
- UniProt ID
- O43617
- Observed MW
- 20 kDa, 30 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab08947(TRAPPC3 antibody) at dilution of 1:800
IP Result of anti-TRAPPC3 (IP:FNab08947, 3ug; Detection:FNab08947 1:800) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human placenta using FNab08947(TRAPPC3 antibody) at dilution of 1:100
- Background
- TRAPPC3(trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines.(15728249, 21273506, 23394947)
