Products
ELOB antibody
- Product Name
- ELOB antibody
- Catalogue No.
- FNab08549
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
- Alternative Names
- Elongin-B (EloB)|Elongin 18 kDa subunit|RNA polymerase II transcription factor SIII subunit B|SIII p18|Transcription elongation factor B polypeptide 2|ELOB|TCEB2 antibody
- UniProt ID
- Q15370
- Observed MW
- 16 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IP: 1:20 - 1:50
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab08549(TCEB2 antibody) at dilution of 1:1000
IP Result of anti-TCEB2 (IP:FNab08549, 4ug; Detection:FNab08549 1:500) with HEK-293 cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab08549(TCEB2 antibody) at dilution of 1:100
- Background
- This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
