SOD2 antibody

Synonyms:Superoxide dismutase [Mn] antibody, mitochondrial(EC:1.15.1.1) antibody, SOD2 antibody
Catalogue No.:FNab08104Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IF, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
SOD2 antibody
Catalogue No.
FNab08104
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
superoxide dismutase 2, mitochondrial
Alternative Names
Superoxide dismutase [Mn] antibody, mitochondrial(EC:1.15.1.1) antibody, SOD2 antibody
UniProt ID
P04179
Observed MW
25 kDa
Application
Tested Applications
ELISA, WB, IF, IHC, IP
Recommended dilution
WB: 1:500-1:5000; IP: 1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab08104(SOD2 antibody) at dilution of 1:1000
IP Result of anti-SOD2 (IP:FNab08104, 4ug; Detection:FNab08104 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08104(SOD2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using FNab08104 (SOD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
Background
SOD2(superoxide dismutase 2, mitochondrial) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.