HeLa cells were subjected to SDS PAGE followed by western blot with FNab08104(SOD2 antibody) at dilution of 1:1000
IP Result of anti-SOD2 (IP:FNab08104, 4ug; Detection:FNab08104 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08104(SOD2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using FNab08104 (SOD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
Background
SOD2(superoxide dismutase 2, mitochondrial) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.