Products
SETD1B antibody
- Product Name
- SETD1B antibody
- Catalogue No.
- FNab07766
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- SET domain containing 1B
- Alternative Names
- KIAA1076 antibody, KMT2G antibody, SET1B antibody
- UniProt ID
- Q9UPS6
- Observed MW
- 260 kDa
Application
- Tested Applications
- ELISA, WB, IP, IHC,IF
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07766(SETD1B antibody) at dilution of 1:1000
IP Result of anti-SETD1B (IP:FNab07766, 5ug; Detection:FNab07766 1:300) with MCF-7 cells lysate 3250ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab07766(SETD1B Antibody) at dilution of 1:50
Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using FNab07766 (SETD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase(HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
